Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4187487..4188141 Replicon chromosome
Accession NZ_CP027221
Organism Escherichia coli strain 2015C-3101

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C6H98_RS22375 Protein ID WP_000244777.1
Coordinates 4187487..4187894 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6H98_RS22380 Protein ID WP_000354046.1
Coordinates 4187875..4188141 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6H98_RS22355 4183444..4185177 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
C6H98_RS22360 4185183..4185893 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6H98_RS22365 4185918..4186814 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6H98_RS22370 4186926..4187447 + 522 WP_001055874.1 flavodoxin FldB -
C6H98_RS22375 4187487..4187894 - 408 WP_000244777.1 toxin CptA Toxin
C6H98_RS22380 4187875..4188141 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6H98_RS22385 4188384..4189364 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
C6H98_RS22390 4189560..4190219 - 660 WP_000250274.1 hemolysin III family protein -
C6H98_RS22395 4190383..4190694 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C6H98_RS22400 4190739..4192172 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
C6H98_RS22405 4192229..4192972 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T97698 WP_000244777.1 NZ_CP027221:c4187894-4187487 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T97698 NZ_CP027221:c4187894-4187487 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT97698 WP_000354046.1 NZ_CP027221:c4188141-4187875 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT97698 NZ_CP027221:c4188141-4187875 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References