Detailed information of TA system TA097695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3710662..3711287
• Replicon: chromosome
• Accession: NZ_CP027221
• Organism: Escherichia coli strain 2015C-3101

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6H98_RS19950
• Protein ID: WP_000911330.1
• Coordinates: 3710662..3711060 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6H98_RS19955
• Protein ID: WP_000450524.1
• Coordinates: 3711060..3711287 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6H98_RS19930	3706540..3706740	+	201	WP_000383836.1	YpfN family protein	-
C6H98_RS19935	3706850..3707548	-	699	WP_000679812.1	esterase	-
C6H98_RS19940	3707622..3709637	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6H98_RS19945	3709652..3710515	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
C6H98_RS19950	3710662..3711060	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6H98_RS19955	3711060..3711287	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6H98_RS19960	3711441..3712154	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6H98_RS19965	3712367..3713401	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C6H98_RS19970	3713418..3714296	-	879	WP_100210193.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6H98_RS19975	3714442..3715026	+	585	WP_000176194.1	glycine cleavage system transcriptional repressor	-
C6H98_RS19980	3715026..3715496	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T97695 WP_000911330.1 NZ_CP027221:c3711060-3710662 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T97695 NZ_CP027221:c3711060-3710662 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT97695 WP_000450524.1 NZ_CP027221:c3711287-3711060 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT97695 NZ_CP027221:c3711287-3711060 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33