Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2526819..2527457 Replicon chromosome
Accession NZ_CP027221
Organism Escherichia coli strain 2015C-3101

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C6H98_RS13500 Protein ID WP_000813794.1
Coordinates 2527281..2527457 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C6H98_RS13495 Protein ID WP_001270286.1
Coordinates 2526819..2527235 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6H98_RS13475 2521971..2522912 - 942 WP_001251315.1 ABC transporter permease -
C6H98_RS13480 2522913..2523926 - 1014 WP_000220394.1 ABC transporter ATP-binding protein -
C6H98_RS13485 2523944..2525089 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
C6H98_RS13490 2525334..2526740 - 1407 WP_000760637.1 PLP-dependent aminotransferase family protein -
C6H98_RS13495 2526819..2527235 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C6H98_RS13500 2527281..2527457 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C6H98_RS13505 2527679..2527909 + 231 WP_000494244.1 YncJ family protein -
C6H98_RS13510 2528001..2529962 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C6H98_RS13515 2530035..2530571 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C6H98_RS13520 2530624..2531832 + 1209 WP_071532666.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2531872..2533020 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T97685 WP_000813794.1 NZ_CP027221:c2527457-2527281 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T97685 NZ_CP027221:c2527457-2527281 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT97685 WP_001270286.1 NZ_CP027221:c2527235-2526819 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT97685 NZ_CP027221:c2527235-2526819 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References