Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 5472040..5472671 Replicon chromosome
Accession NZ_CP027219
Organism Escherichia coli strain 2015C-3163

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag C6H73_RS29455 Protein ID WP_001259385.1
Coordinates 5472040..5472315 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag C6H73_RS29460 Protein ID WP_000593554.1
Coordinates 5472312..5472671 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6H73_RS29440 5467044..5468111 + 1068 WP_000361467.1 HlyD family secretion protein -
C6H73_RS29445 5468108..5470843 + 2736 WP_000149167.1 ribosome-associated ATPase/putative transporter RbbA -
C6H73_RS29450 5470843..5471967 + 1125 WP_001216257.1 ABC transporter permease -
C6H73_RS29455 5472040..5472315 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
C6H73_RS29460 5472312..5472671 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6H73_RS29465 5472721..5473581 - 861 WP_001131783.1 class II aldolase -
C6H73_RS29470 5473613..5473882 - 270 WP_000933060.1 HPr family phosphocarrier protein -
C6H73_RS29475 5473872..5475380 - 1509 WP_001298718.1 carbohydrate kinase -
C6H73_RS29480 5475373..5476731 - 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
C6H73_RS29485 5476808..5477089 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
C6H73_RS29490 5477086..5477559 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5464423..5472671 8248


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T97669 WP_001259385.1 NZ_CP027219:5472040-5472315 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T97669 NZ_CP027219:5472040-5472315 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT97669 WP_000593554.1 NZ_CP027219:5472312-5472671 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT97669 NZ_CP027219:5472312-5472671 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References