Detailed information of TA system TA097664

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 4477027..4477802
• Replicon: chromosome
• Accession: NZ_CP027219
• Organism: Escherichia coli strain 2015C-3163

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7V7JG91
• Locus tag: C6H73_RS24470
• Protein ID: WP_001193488.1
• Coordinates: 4477027..4477398 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7V7JFU6
• Locus tag: C6H73_RS24475
• Protein ID: WP_001059301.1
• Coordinates: 4477437..4477802 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6H73_RS24440	4472977..4473693	+	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-
C6H73_RS24445	4473713..4474819	+	1107	WP_001298927.1	N-acetylneuraminate epimerase	-
C6H73_RS24450	4474884..4475864	+	981	WP_000991444.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
C6H73_RS24470	4477027..4477398	-	372	WP_001193488.1	TA system toxin CbtA family protein	Toxin
C6H73_RS24475	4477437..4477802	-	366	WP_001059301.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6H73_RS24480	4477828..4478049	-	222	WP_000691983.1	DUF987 domain-containing protein	-
C6H73_RS24485	4478046..4478588	-	543	WP_015740440.1	DNA repair protein RadC	-
C6H73_RS24490	4478601..4479044	-	444	WP_001096016.1	antirestriction protein	-
C6H73_RS24495	4479075..4479896	-	822	WP_001234408.1	DUF945 domain-containing protein	-
C6H73_RS24500	4480043..4480489	-	447	WP_032162830.1	hypothetical protein	-
C6H73_RS24505	4480561..4481013	-	453	WP_000734321.1	hypothetical protein	-
C6H73_RS24510	4481049..4481765	-	717	WP_000174910.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	4469845..4484543	14698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13832.94 Da
• Isoelectric Point: 7.2419

>T97664 WP_001193488.1 NZ_CP027219:c4477398-4477027 [Escherichia coli]
MQTISSHPTRATQPCLSPVEIWQRLLTCLLSQHYGLTLNDTPFSNETTILEHIDAGVSLCDAVNFLVEKYELVRIDCNDC
SVMEKSPFITSIDILRARKASGLMKRNSHKTVTRVTAGRLQEL

Nucleotide

• Length: 372 bp

>T97664 NZ_CP027219:c4477398-4477027 [Escherichia coli]
ATGCAAACTATATCATCACACCCGACACGGGCGACTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGCGGTTACTGAC
TTGTCTGTTGTCGCAGCACTATGGTCTGACGTTGAATGACACACCGTTCAGTAATGAAACCACCATACTGGAACACATTG
ATGCTGGAGTATCGCTGTGCGATGCGGTGAACTTTCTTGTTGAAAAATATGAACTGGTAAGAATTGACTGTAATGATTGC
TCTGTCATGGAGAAGTCACCGTTCATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACGCAA
CAGTCACAAAACTGTTACCAGGGTAACGGCAGGTCGACTTCAGGAGCTATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13388.25 Da
• Isoelectric Point: 5.9530

>AT97664 WP_001059301.1 NZ_CP027219:c4477802-4477437 [Escherichia coli]
MNNHSESGTKPENPACQQWGLKCAITPCFGARLVQEGNRLHFLSDRAGFSGAFAVDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHPHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT97664 NZ_CP027219:c4477802-4477437 [Escherichia coli]
ATGAACAATCACTCAGAATCCGGTACAAAGCCGGAGAATCCCGCCTGCCAGCAGTGGGGGCTTAAATGTGCTATTACCCC
CTGTTTTGGTGCCCGCCTGGTACAGGAGGGCAACCGATTGCATTTTCTCTCTGACCGCGCAGGATTTAGCGGGGCATTTG
CCGTCGATGTTGCAATGCGCCTTGACCAGGCATTTCCGTTAATGATGAAGCAACTTGAGCTGATGCTGACCAGTGGAGAG
CTTAACCCTCGCCACCCACACTGCGTCACGCTTTACCACAATGGACTCACCTGTGAAGCCGATACACTAGGCTCCTGTGG
TTATGTCTACATTGCTATCTATCCTGAGCAAACTGAGCCGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A7V7JG91

Antitoxin

• AlphaFold DB: A0A7V7JFU6