Detailed information of TA system TA097660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3228212..3228690
• Replicon: chromosome
• Accession: NZ_CP027219
• Organism: Escherichia coli strain 2015C-3163

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: C6H73_RS18010
• Protein ID: WP_001303876.1
• Coordinates: 3228403..3228690 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: C6H73_RS18005
• Protein ID: WP_000536233.1
• Coordinates: 3228212..3228403 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6H73_RS17970	3223591..3224361	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
C6H73_RS17975	3224443..3225129	-	687	WP_000788749.1	ATP-binding protein	-
C6H73_RS17980	3225136..3226252	-	1117	Protein_3277	DNA-binding protein	-
C6H73_RS17985	3226331..3226786	+	456	WP_000273724.1	hypothetical protein	-
C6H73_RS17990	3226993..3227418	-	426	WP_000693855.1	Rha family transcriptional regulator	-
C6H73_RS17995	3227402..3227674	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
C6H73_RS18000	3227783..3228184	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
C6H73_RS18005	3228212..3228403	+	192	WP_000536233.1	hypothetical protein	Antitoxin
C6H73_RS18010	3228403..3228690	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6H73_RS18015	3228967..3229122	+	156	WP_000379575.1	DUF1391 family protein	-
C6H73_RS18025	3229264..3229653	+	390	WP_000394513.1	hypothetical protein	-
C6H73_RS18030	3229840..3230025	-	186	WP_001133046.1	hypothetical protein	-
C6H73_RS18050	3231866..3232054	+	189	WP_000413705.1	cell division inhibition protein DicB	-
C6H73_RS18055	3232051..3232242	+	192	WP_001098307.1	DUF1482 family protein	-
C6H73_RS18060	3232336..3233022	+	687	WP_199852804.1	exonuclease VIII	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3220679..3247285	26606

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T97660 WP_001303876.1 NZ_CP027219:3228403-3228690 [Escherichia coli]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T97660 NZ_CP027219:3228403-3228690 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT97660 WP_000536233.1 NZ_CP027219:3228212-3228403 [Escherichia coli]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT97660 NZ_CP027219:3228212-3228403 [Escherichia coli]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA