Detailed information of TA system TA097647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1193240..1193865
• Replicon: chromosome
• Accession: NZ_CP027219
• Organism: Escherichia coli strain 2015C-3163

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6H73_RS06395
• Protein ID: WP_000911330.1
• Coordinates: 1193467..1193865 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6H73_RS06390
• Protein ID: WP_000450524.1
• Coordinates: 1193240..1193467 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6H73_RS06365	1189042..1189512	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C6H73_RS06370	1189512..1190084	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C6H73_RS06375	1190230..1191108	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6H73_RS06380	1191125..1192159	+	1035	WP_001368798.1	outer membrane protein assembly factor BamC	-
C6H73_RS06385	1192372..1193085	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6H73_RS06390	1193240..1193467	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6H73_RS06395	1193467..1193865	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6H73_RS06400	1194012..1194875	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
C6H73_RS06405	1194890..1196905	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6H73_RS06410	1196979..1197677	+	699	WP_000679812.1	esterase	-
C6H73_RS06415	1197787..1197987	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T97647 WP_000911330.1 NZ_CP027219:1193467-1193865 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T97647 NZ_CP027219:1193467-1193865 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT97647 WP_000450524.1 NZ_CP027219:1193240-1193467 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT97647 NZ_CP027219:1193240-1193467 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33