Detailed information of TA system TA097635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 595486..596123
• Replicon: chromosome
• Accession: NZ_CP027218
• Organism: Pseudomonas sp. DTU12.3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C2E19_RS02660
• Protein ID: WP_129387720.1
• Coordinates: 595486..595893 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: C2E19_RS02665
• Protein ID: WP_129387723.1
• Coordinates: 595893..596123 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2E19_RS02640	591163..591855	-	693	WP_129387713.1	ABC transporter permease	-
C2E19_RS02645	591934..592683	-	750	WP_034153033.1	ABC transporter substrate-binding protein	-
C2E19_RS02650	592697..593470	-	774	WP_007909167.1	ABC transporter ATP-binding protein	-
C2E19_RS02655	594024..595415	+	1392	WP_129387717.1	GABA permease	-
C2E19_RS02660	595486..595893	-	408	WP_129387720.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C2E19_RS02665	595893..596123	-	231	WP_129387723.1	antitoxin	Antitoxin
C2E19_RS02670	596288..596740	-	453	WP_129387726.1	hypothetical protein	-
C2E19_RS02675	596747..597160	-	414	WP_053116577.1	hypothetical protein	-
C2E19_RS02680	597190..598164	-	975	WP_129387730.1	alpha/beta fold hydrolase	-
C2E19_RS02685	598233..599315	-	1083	WP_129387733.1	DUF1016 family protein	-
C2E19_RS02690	599575..600024	+	450	WP_129387736.1	carboxypeptidase regulatory-like domain-containing protein	-
C2E19_RS02695	600105..600452	-	348	WP_129387739.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	584918..596123	11205

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14928.32 Da
• Isoelectric Point: 6.2241

>T97635 WP_129387720.1 NZ_CP027218:c595893-595486 [Pseudomonas sp. DTU12.3]
MIKYMLDTNICIFTIKNKPQIVREAFNQHSGQLCISAITLMELIYGAEKSASPEKNLAIVEGFVARLEVLPFDNDAAAQT
GMIRSELAKAGTPIGPYDQMIAGHARSLGLIVVTNNVREFERVSGLRIEDWVHPV

Nucleotide

• Length: 408 bp

>T97635 NZ_CP027218:c595893-595486 [Pseudomonas sp. DTU12.3]
ATGATCAAATACATGCTGGATACCAACATCTGCATCTTCACCATCAAAAACAAACCACAAATTGTCCGGGAGGCCTTCAA
TCAACATTCCGGGCAGTTGTGCATCAGCGCTATCACGCTTATGGAGCTTATTTACGGCGCTGAAAAGTCGGCGTCCCCTG
AAAAGAATCTAGCGATTGTCGAAGGATTCGTTGCGCGATTGGAGGTCTTGCCTTTCGACAATGACGCCGCAGCACAGACC
GGTATGATCCGGTCTGAACTGGCGAAAGCTGGAACACCGATTGGGCCGTACGACCAAATGATCGCAGGCCACGCGCGGTC
GCTTGGACTTATCGTAGTCACGAACAATGTGCGGGAATTTGAACGTGTTTCCGGATTGCGTATTGAGGATTGGGTTCATC
CCGTTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8559.58 Da
• Isoelectric Point: 4.3719

>AT97635 WP_129387723.1 NZ_CP027218:c596123-595893 [Pseudomonas sp. DTU12.3]
MEQTTIFMSNRSQAVRLPKAVAMPGDVKRVEVIAIGRTRVITPAGESWDSWFDGDSVTPDFMADREQPADQERESF

Nucleotide

• Length: 231 bp

>AT97635 NZ_CP027218:c596123-595893 [Pseudomonas sp. DTU12.3]
ATGGAACAGACCACAATTTTCATGAGCAATAGAAGTCAGGCAGTTCGACTGCCAAAGGCAGTTGCGATGCCCGGCGACGT
TAAACGAGTGGAAGTCATCGCGATTGGCAGAACACGCGTCATTACGCCTGCTGGAGAAAGCTGGGATAGCTGGTTTGATG
GCGACAGCGTCACACCTGATTTCATGGCTGACCGTGAGCAACCTGCTGATCAGGAGCGCGAGTCGTTCTGA