Detailed information of TA system TA097619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1345522..1346147
• Replicon: chromosome
• Accession: NZ_CP027205
• Organism: Escherichia coli strain WCHEC025943

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CXB56_RS09985
• Protein ID: WP_000911330.1
• Coordinates: 1345749..1346147 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CXB56_RS09980
• Protein ID: WP_000450524.1
• Coordinates: 1345522..1345749 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXB56_RS09955	1341325..1341795	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CXB56_RS09960	1341795..1342367	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CXB56_RS09965	1342513..1343391	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CXB56_RS09970	1343408..1344442	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CXB56_RS09975	1344655..1345368	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CXB56_RS09980	1345522..1345749	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CXB56_RS09985	1345749..1346147	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CXB56_RS09990	1346294..1347157	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
CXB56_RS09995	1347172..1349187	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
CXB56_RS10000	1349261..1349602	+	342	Protein_1282	esterase	-
CXB56_RS10005	1349684..1350430	-	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T97619 WP_000911330.1 NZ_CP027205:1345749-1346147 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T97619 NZ_CP027205:1345749-1346147 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT97619 WP_000450524.1 NZ_CP027205:1345522-1345749 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT97619 NZ_CP027205:1345522-1345749 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33