Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 70351..70876 Replicon plasmid p2_025943
Accession NZ_CP027200
Organism Escherichia coli strain WCHEC025943

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CXB56_RS01065 Protein ID WP_001159871.1
Coordinates 70351..70656 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CXB56_RS01070 Protein ID WP_000813634.1
Coordinates 70658..70876 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXB56_RS01035 66093..67118 + 1026 WP_000864945.1 zinc-binding alcohol dehydrogenase family protein -
CXB56_RS01040 67177..67848 - 672 WP_046788564.1 FadR family transcriptional regulator -
CXB56_RS01045 67943..68481 - 539 Protein_82 GGDEF domain-containing protein -
CXB56_RS01050 68492..69189 - 698 Protein_83 IS1 family transposase -
CXB56_RS01055 69241..69369 - 129 Protein_84 colicin M immunity protein -
CXB56_RS01060 69541..70350 - 810 WP_000016963.1 site-specific integrase -
CXB56_RS01065 70351..70656 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CXB56_RS01070 70658..70876 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CXB56_RS01085 71344..72039 + 696 WP_072652234.1 DNA-binding protein -
CXB56_RS01090 72036..72656 + 621 WP_072652229.1 hypothetical protein -
CXB56_RS01095 72653..73441 + 789 WP_072652230.1 DUF2971 domain-containing protein -
CXB56_RS01100 73477..74217 + 741 WP_072652231.1 abortive infection family protein -
CXB56_RS01105 74376..75005 - 630 Protein_92 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / rmtB / blaTEM-1B / aac(3)-IId / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / tet(A) - 1..75779 75779


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T97610 WP_001159871.1 NZ_CP027200:c70656-70351 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T97610 NZ_CP027200:c70656-70351 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT97610 WP_000813634.1 NZ_CP027200:c70876-70658 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT97610 NZ_CP027200:c70876-70658 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References