Detailed information of TA system TA097609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 65864..66465
• Replicon: plasmid p1_025943
• Accession: NZ_CP027199
• Organism: Escherichia coli strain WCHEC025943

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: CXB56_RS00350
• Protein ID: WP_001216034.1
• Coordinates: 65864..66244 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: CXB56_RS00355
• Protein ID: WP_001190712.1
• Coordinates: 66244..66465 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXB56_RS00325	61304..62788	-	1485	WP_089046482.1	terminase	-
CXB56_RS00330	62788..63981	-	1194	WP_000219605.1	terminase	-
CXB56_RS00335	64068..64520	-	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
CXB56_RS00340	64609..65652	-	1044	WP_000648824.1	DUF968 domain-containing protein	-
CXB56_RS00345	65680..65859	-	180	WP_000113018.1	hypothetical protein	-
CXB56_RS00350	65864..66244	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CXB56_RS00355	66244..66465	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CXB56_RS00360	66538..66927	-	390	WP_000506726.1	DNA repair protein	-
CXB56_RS00365	67051..67302	-	252	WP_001283837.1	DNA polymerase III subunit theta	-
CXB56_RS00370	67476..67685	+	210	WP_001344848.1	hypothetical protein	-
CXB56_RS27505	67670..67942	-	273	WP_176398880.1	antitoxin PHD	-
CXB56_RS00375	67916..68527	-	612	Protein_70	hypothetical protein	-
CXB56_RS00380	68509..68883	-	375	WP_033553822.1	hypothetical protein	-
CXB56_RS00385	68890..69183	-	294	WP_033553821.1	hypothetical protein	-
CXB56_RS00390	69362..69595	-	234	WP_000517420.1	hypothetical protein	-
CXB56_RS00395	69672..69932	-	261	WP_033553819.1	hypothetical protein	-
CXB56_RS00400	69929..70726	-	798	WP_100728230.1	DUF551 domain-containing protein	-
CXB56_RS00405	70728..71372	-	645	WP_100728229.1	ead/Ea22-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95895	95895

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T97609 WP_001216034.1 NZ_CP027199:c66244-65864 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T97609 NZ_CP027199:c66244-65864 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT97609 WP_001190712.1 NZ_CP027199:c66465-66244 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT97609 NZ_CP027199:c66465-66244 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6