Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 807131..807756 Replicon chromosome
Accession NZ_CP027189
Organism Klebsiella pneumoniae strain KPHS1249

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag C5Z24_RS04025 Protein ID WP_012737091.1
Coordinates 807131..807514 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag C5Z24_RS04030 Protein ID WP_004150355.1
Coordinates 807514..807756 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5Z24_RS04010 804497..805399 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
C5Z24_RS04015 805396..806031 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
C5Z24_RS04020 806028..806957 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
C5Z24_RS04025 807131..807514 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
C5Z24_RS04030 807514..807756 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
C5Z24_RS04035 807961..808878 + 918 WP_012737090.1 alpha/beta hydrolase -
C5Z24_RS04040 808892..809833 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
C5Z24_RS04045 809878..810315 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
C5Z24_RS04050 810312..811172 - 861 WP_004146232.1 virulence factor BrkB family protein -
C5Z24_RS04055 811166..811765 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T97594 WP_012737091.1 NZ_CP027189:c807514-807131 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T97594 NZ_CP027189:c807514-807131 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT97594 WP_004150355.1 NZ_CP027189:c807756-807514 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT97594 NZ_CP027189:c807756-807514 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References