Detailed information of TA system TA097584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 37793..38377
• Replicon: plasmid unnamed1
• Accession: NZ_CP027185
• Organism: Acinetobacter baumannii strain AR_0052

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N9JY46
• Locus tag: CSB68_RS20555
• Protein ID: WP_000286963.1
• Coordinates: 38075..38377 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CSB68_RS20550
• Protein ID: WP_000985610.1
• Coordinates: 37793..38074 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB68_RS20525	32915..33778	-	864	WP_000991898.1	hypothetical protein	-
CSB68_RS20530	34026..34340	-	315	WP_000582285.1	hypothetical protein	-
CSB68_RS20535	34394..35170	-	777	WP_000698343.1	hypothetical protein	-
CSB68_RS20540	35818..36579	-	762	Protein_32	ATP-binding protein	-
CSB68_RS20545	36705..37565	-	861	WP_002009063.1	IS5 family transposase	-
CSB68_RS20550	37793..38074	-	282	WP_000985610.1	putative addiction module antidote protein	Antitoxin
CSB68_RS20555	38075..38377	-	303	WP_000286963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB68_RS20560	38539..39078	-	540	WP_002055813.1	hypothetical protein	-
CSB68_RS20565	39103..39942	-	840	WP_001058024.1	alpha/beta fold hydrolase	-
CSB68_RS20570	40035..40625	+	591	WP_000058346.1	TetR/AcrR family transcriptional regulator	-
CSB68_RS20575	40944..41939	+	996	WP_001038515.1	zinc-dependent alcohol dehydrogenase family protein	-
CSB68_RS20580	41929..42945	+	1017	WP_086379304.1	zinc-dependent alcohol dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..105128	105128
	-	flank	IS/Tn	-	-	36705..37565	860

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11545.02 Da
• Isoelectric Point: 4.6838

>T97584 WP_000286963.1 NZ_CP027185:c38377-38075 [Acinetobacter baumannii]
MYSIYTTEAFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T97584 NZ_CP027185:c38377-38075 [Acinetobacter baumannii]
ATGTACTCAATATATACCACAGAAGCCTTTGATGATTGGTTTACCAAGTTAAAAGATCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGGAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTTTCTGAGTTACGTTTCT
TCTTTGGACCTGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTCGTTATTCTCTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT97584 WP_000985610.1 NZ_CP027185:c38074-37793 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT97584 NZ_CP027185:c38074-37793 [Acinetobacter baumannii]
ATGGCTATCAAACTTCGTAAATGGGATAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCTGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A836MGN2