Detailed information of TA system TA097581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 39156..39740
• Replicon: plasmid unnamed4
• Accession: NZ_CP027182
• Organism: Acinetobacter baumannii strain AR_0070

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N9JY46
• Locus tag: CSB70_RS21130
• Protein ID: WP_000286963.1
• Coordinates: 39438..39740 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CSB70_RS21125
• Protein ID: WP_000985610.1
• Coordinates: 39156..39437 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB70_RS21100	34278..35141	-	864	WP_000991898.1	hypothetical protein	-
CSB70_RS21105	35389..35703	-	315	WP_000582285.1	hypothetical protein	-
CSB70_RS21110	35757..36533	-	777	WP_000698343.1	hypothetical protein	-
CSB70_RS21115	37181..37942	-	762	Protein_35	ATP-binding protein	-
CSB70_RS21120	38068..38928	-	861	WP_002009063.1	IS5 family transposase	-
CSB70_RS21125	39156..39437	-	282	WP_000985610.1	putative addiction module antidote protein	Antitoxin
CSB70_RS21130	39438..39740	-	303	WP_000286963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB70_RS21135	39902..40441	-	540	WP_002055813.1	hypothetical protein	-
CSB70_RS21140	40466..41304	-	839	Protein_40	alpha/beta fold hydrolase	-
CSB70_RS21145	41397..41987	+	591	WP_000058346.1	TetR/AcrR family transcriptional regulator	-
CSB70_RS21725	42277..42372	-	96	Protein_42	DNA-binding protein	-
CSB70_RS21155	42370..42591	-	222	Protein_43	IS3 family transposase	-
CSB70_RS21730	42726..43010	-	285	Protein_44	antibiotic biosynthesis monooxygenase	-
CSB70_RS21165	43031..44029	-	999	WP_000649019.1	zinc-binding alcohol dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..105127	105127
	-	flank	IS/Tn	-	-	38068..38928	860

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11545.02 Da
• Isoelectric Point: 4.6838

>T97581 WP_000286963.1 NZ_CP027182:c39740-39438 [Acinetobacter baumannii]
MYSIYTTEAFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T97581 NZ_CP027182:c39740-39438 [Acinetobacter baumannii]
ATGTACTCAATATATACCACAGAAGCCTTTGATGATTGGTTTACCAAGTTAAAAGATCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGGAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTTTCTGAGTTACGTTTCT
TCTTTGGACCTGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTCGTTATTCTCTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT97581 WP_000985610.1 NZ_CP027182:c39437-39156 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT97581 NZ_CP027182:c39437-39156 [Acinetobacter baumannii]
ATGGCTATCAAACTTCGTAAATGGGATAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCTGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A836MGN2