Detailed information of TA system TA097561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 52723..53273
• Replicon: plasmid unnamed1
• Accession: NZ_CP027175
• Organism: Pseudomonas aeruginosa strain AR_0230

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CSB85_RS33740
• Protein ID: WP_019727125.1
• Coordinates: 52723..52998 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A6B1YL09
• Locus tag: CSB85_RS33745
• Protein ID: WP_019727126.1
• Coordinates: 52998..53273 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB85_RS33705	48489..48791	+	303	WP_023131919.1	anti-CRISPR protein AcrE1	-
CSB85_RS33710	48814..49227	+	414	WP_048909912.1	anti-CRISPR protein AcrF3	-
CSB85_RS33715	49240..49479	+	240	WP_044069671.1	anti-CRISPR protein	-
CSB85_RS34180	49775..50071	+	297	WP_108084449.1	hypothetical protein	-
CSB85_RS33725	50524..51003	+	480	WP_048909913.1	hypothetical protein	-
CSB85_RS33730	51060..52235	+	1176	WP_177281057.1	hypothetical protein	-
CSB85_RS33735	52216..52632	+	417	WP_048909914.1	hypothetical protein	-
CSB85_RS33740	52723..52998	-	276	WP_019727125.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB85_RS33745	52998..53273	-	276	WP_019727126.1	ribbon-helix-helix domain-containing protein	Antitoxin
CSB85_RS33750	53545..53931	+	387	WP_048909915.1	hypothetical protein	-
CSB85_RS34185	54126..54284	+	159	WP_153576009.1	hypothetical protein	-
CSB85_RS33760	54608..54916	+	309	WP_019727129.1	hypothetical protein	-
CSB85_RS33775	55566..56018	+	453	WP_019727132.1	DNA repair protein RadC	-
CSB85_RS33785	56456..56704	+	249	WP_019727134.1	hypothetical protein	-
CSB85_RS33790	56754..56948	-	195	WP_171978759.1	hypothetical protein	-
CSB85_RS33795	57064..57345	+	282	WP_048909916.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71782	71782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-48)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10779.36 Da
• Isoelectric Point: 7.1659

>T97561 WP_019727125.1 NZ_CP027175:c52998-52723 [Pseudomonas aeruginosa]
MELKWTSKGLSDVARLYEFLATVNREAAARTVQQLTSAPTALLTNPRLGERLEEFEPRDVRRILIGHYEMRYEILDSCIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T97561 NZ_CP027175:c52998-52723 [Pseudomonas aeruginosa]
ATGGAGTTGAAGTGGACGAGCAAAGGGCTCTCCGACGTGGCACGGCTCTACGAGTTCCTGGCTACGGTGAACAGGGAAGC
TGCCGCCCGCACGGTGCAGCAGCTGACGAGCGCGCCGACCGCCTTGTTGACCAACCCGCGGCTGGGCGAGCGCCTGGAAG
AGTTCGAACCGCGGGATGTTCGCCGCATCCTGATCGGCCACTATGAGATGCGCTATGAGATCCTGGATTCCTGCATCTAC
GTGCTTCGTCTGTGGCACACACGCGAAGACAGGTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10142.32 Da
• Isoelectric Point: 4.8433

>AT97561 WP_019727126.1 NZ_CP027175:c53273-52998 [Pseudomonas aeruginosa]
MSTAAKPTRTVTAHVPVDLAERVDAIAERLERSKNWIVRQALSAWIDQEDERSRLTREALDDVDAGRVVDHQAVQAWAES
LGTDSPLPVPR

Nucleotide

• Length: 276 bp

>AT97561 NZ_CP027175:c53273-52998 [Pseudomonas aeruginosa]
ATGAGCACAGCAGCCAAACCAACGCGCACCGTGACTGCTCACGTCCCGGTAGATCTGGCCGAGCGCGTCGATGCAATCGC
CGAGCGCCTGGAGCGATCGAAGAACTGGATCGTCAGGCAGGCGCTGTCAGCCTGGATCGACCAGGAAGACGAGCGTAGCC
GTCTGACACGTGAAGCCTTGGATGATGTAGACGCAGGACGTGTCGTCGACCACCAGGCCGTCCAGGCCTGGGCCGAAAGC
CTGGGCACTGACTCTCCGCTGCCGGTACCACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6B1YL09