Detailed information of TA system TA097558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4207496..4208082
• Replicon: chromosome
• Accession: NZ_CP027174
• Organism: Pseudomonas aeruginosa strain AR_0230

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: CSB85_RS20195
• Protein ID: WP_003120987.1
• Coordinates: 4207783..4208082 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CSB85_RS20190
• Protein ID: WP_003448662.1
• Coordinates: 4207496..4207786 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB85_RS20175	4203078..4204967	+	1890	WP_003448700.1	hypothetical protein	-
CSB85_RS20180	4204964..4206940	+	1977	WP_010792226.1	DEAD/DEAH box helicase	-
CSB85_RS20185	4207081..4207425	+	345	WP_003448665.1	hypothetical protein	-
CSB85_RS20190	4207496..4207786	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
CSB85_RS20195	4207783..4208082	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB85_RS20200	4208284..4209405	+	1122	WP_003448658.1	TcpQ domain-containing protein	-
CSB85_RS20205	4209405..4211114	+	1710	WP_010792227.1	PilN family type IVB pilus formation outer membrane protein	-
CSB85_RS20210	4211118..4212443	+	1326	WP_003120992.1	type 4b pilus protein PilO2	-
CSB85_RS20215	4212433..4212966	+	534	WP_003120993.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4178092..4265187	87095

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T97558 WP_003120987.1 NZ_CP027174:c4208082-4207783 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T97558 NZ_CP027174:c4208082-4207783 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT97558 WP_003448662.1 NZ_CP027174:c4207786-4207496 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT97558 NZ_CP027174:c4207786-4207496 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73