Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 1816724..1817332 Replicon chromosome
Accession NZ_CP027172
Organism Pseudomonas aeruginosa strain AR_0353

Toxin (Protein)


Gene name PfiT Uniprot ID Q9I5J9
Locus tag CSB90_RS08565 Protein ID WP_003114156.1
Coordinates 1816985..1817332 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag CSB90_RS08560 Protein ID WP_003114155.1
Coordinates 1816724..1816975 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSB90_RS08540 1812363..1812719 + 357 WP_003114150.1 DUF2523 domain-containing protein -
CSB90_RS08545 1812723..1814006 + 1284 WP_023127773.1 hypothetical protein -
CSB90_RS08550 1814236..1815528 + 1293 WP_003134394.1 hypothetical protein -
CSB90_RS08555 1815528..1816510 + 983 Protein_1653 tyrosine-type recombinase/integrase -
CSB90_RS08560 1816724..1816975 + 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CSB90_RS08565 1816985..1817332 + 348 WP_003114156.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CSB90_RS08575 1817659..1818561 - 903 WP_003085447.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
CSB90_RS08580 1819013..1819750 - 738 WP_003124252.1 murein L,D-transpeptidase catalytic domain family protein -
CSB90_RS08585 1819830..1820873 - 1044 WP_003134392.1 L,D-transpeptidase -
CSB90_RS08590 1821009..1821701 - 693 WP_003085458.1 16S rRNA pseudouridine(516) synthase -
CSB90_RS08595 1821701..1821973 - 273 WP_003134390.1 cysteine-rich CWC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1798850..1817332 18482


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12984.78 Da        Isoelectric Point: 4.4212

>T97545 WP_003114156.1 NZ_CP027172:1816985-1817332 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T97545 NZ_CP027172:1816985-1817332 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT97545 WP_003114155.1 NZ_CP027172:1816724-1816975 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT97545 NZ_CP027172:1816724-1816975 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I5J9

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References