Detailed information of TA system TA097533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3867500..3868140
• Replicon: chromosome
• Accession: NZ_CP027166
• Organism: Pseudomonas aeruginosa strain AR_0357

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CSB94_RS18740
• Protein ID: WP_003134109.1
• Coordinates: 3867730..3868140 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CSB94_RS18735
• Protein ID: WP_031634724.1
• Coordinates: 3867500..3867730 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB94_RS18720	3862698..3864587	-	1890	WP_003105732.1	hypothetical protein	-
CSB94_RS18725	3864808..3865017	-	210	WP_003105733.1	cold-shock protein	-
CSB94_RS18730	3865325..3867244	-	1920	WP_004352838.1	type I DNA topoisomerase	-
CSB94_RS18735	3867500..3867730	+	231	WP_031634724.1	antitoxin	Antitoxin
CSB94_RS18740	3867730..3868140	+	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CSB94_RS18745	3868156..3868353	+	198	WP_023083219.1	hypothetical protein	-
CSB94_RS18750	3868367..3868585	+	219	WP_023083218.1	hypothetical protein	-
CSB94_RS18755	3868651..3868818	-	168	WP_128729268.1	CrpP family protein	-
CSB94_RS18760	3868974..3869462	-	489	WP_004352840.1	single-stranded DNA-binding protein	-
CSB94_RS18765	3869492..3870331	-	840	WP_003105750.1	Rha family transcriptional regulator	-
CSB94_RS18770	3870378..3870926	-	549	WP_004352841.1	DUF3158 family protein	-
CSB94_RS18775	3870932..3871660	-	729	WP_004352842.1	TIGR03761 family integrating conjugative element protein	-
CSB94_RS18780	3871817..3872014	-	198	WP_019396765.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3795236..3886516	91280

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T97533 WP_003134109.1 NZ_CP027166:3867730-3868140 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T97533 NZ_CP027166:3867730-3868140 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACTATCAAGAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATCGCCGGACACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCACCC
CAATCGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT97533 WP_031634724.1 NZ_CP027166:3867500-3867730 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT97533 NZ_CP027166:3867500-3867730 [Pseudomonas aeruginosa]
GTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCTTCCTGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGTTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA