Detailed information of TA system TA097528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3966578..3967218
• Replicon: chromosome
• Accession: NZ_CP027165
• Organism: Pseudomonas aeruginosa strain AR_0360

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CSB97_RS18740
• Protein ID: WP_003105740.1
• Coordinates: 3966578..3966988 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6X2S2
• Locus tag: CSB97_RS18745
• Protein ID: WP_003158175.1
• Coordinates: 3966988..3967218 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB97_RS18695	3962420..3962617	+	198	WP_003105756.1	hypothetical protein	-
CSB97_RS18700	3962773..3963501	+	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
CSB97_RS18705	3963507..3964055	+	549	WP_003105753.1	DUF3158 family protein	-
CSB97_RS18710	3964102..3964941	+	840	WP_003105750.1	Rha family transcriptional regulator	-
CSB97_RS18715	3964971..3965459	+	489	WP_003105748.1	single-stranded DNA-binding protein	-
CSB97_RS18720	3965567..3965812	+	246	WP_023102333.1	CrpP family protein	-
CSB97_RS18725	3965878..3966096	-	219	WP_003105747.1	hypothetical protein	-
CSB97_RS18735	3966296..3966556	-	261	WP_003105742.1	hypothetical protein	-
CSB97_RS18740	3966578..3966988	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CSB97_RS18745	3966988..3967218	-	231	WP_003158175.1	antitoxin	Antitoxin
CSB97_RS18750	3967474..3969393	+	1920	WP_004352838.1	type I DNA topoisomerase	-
CSB97_RS18755	3969701..3969910	+	210	WP_003105733.1	cold-shock protein	-
CSB97_RS18760	3970131..3972020	+	1890	WP_003109354.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3947007..4046995	99988

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T97528 WP_003105740.1 NZ_CP027165:c3966988-3966578 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T97528 NZ_CP027165:c3966988-3966578 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATTGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGACTGGGTCGTCC
CAATCGTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.41 Da
• Isoelectric Point: 4.2821

>AT97528 WP_003158175.1 NZ_CP027165:c3967218-3966988 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVVVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT97528 NZ_CP027165:c3967218-3966988 [Pseudomonas aeruginosa]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGTTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q6X2S2