Detailed information of TA system TA097523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 91041..91777
• Replicon: plasmid unnamed1
• Accession: NZ_CP027161
• Organism: Klebsiella pneumoniae strain AR_0361

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: CSB98_RS27650
• Protein ID: WP_003026803.1
• Coordinates: 91295..91777 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: CSB98_RS27645
• Protein ID: WP_003026799.1
• Coordinates: 91041..91307 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB98_RS27600	87103..87465	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
CSB98_RS27605	87515..87865	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
CSB98_RS27610	88223..88492	+	270	WP_004152102.1	hypothetical protein	-
CSB98_RS27615	88480..89055	+	576	WP_004152103.1	hypothetical protein	-
CSB98_RS27620	89086..89580	+	495	WP_004152104.1	DNA-binding protein	-
CSB98_RS27625	89624..89992	+	369	WP_004152105.1	hypothetical protein	-
CSB98_RS27630	90026..90229	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
CSB98_RS27635	90278..90535	+	258	WP_004152107.1	hypothetical protein	-
CSB98_RS27640	90611..90865	+	255	WP_004152108.1	hypothetical protein	-
CSB98_RS27645	91041..91307	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
CSB98_RS27650	91295..91777	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
CSB98_RS27655	91989..93335	+	1347	WP_020314316.1	ISNCY family transposase	-
CSB98_RS27660	93836..95090	-	1255	Protein_107	IS3 family transposase	-
CSB98_RS27665	95178..96140	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1	-	1..207543	207543

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T97523 WP_003026803.1 NZ_CP027161:91295-91777 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T97523 NZ_CP027161:91295-91777 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT97523 WP_003026799.1 NZ_CP027161:91041-91307 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT97523 NZ_CP027161:91041-91307 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66