Detailed information of TA system TA097504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 19554..20197
• Replicon: plasmid unnamed2
• Accession: NZ_CP027158
• Organism: Klebsiella pneumoniae strain AR_0361

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: CSB98_RS00280
• Protein ID: WP_001044770.1
• Coordinates: 19781..20197 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: CSB98_RS00275
• Protein ID: WP_001261282.1
• Coordinates: 19554..19784 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB98_RS00230	14752..15225	+	474	WP_004152341.1	YkgJ family cysteine cluster protein	-
CSB98_RS00240	16439..17221	-	783	WP_004152340.1	site-specific integrase	-
CSB98_RS00245	17221..17553	-	333	WP_004152339.1	hypothetical protein	-
CSB98_RS00250	17560..17958	-	399	WP_004171440.1	hypothetical protein	-
CSB98_RS00255	17984..18313	-	330	WP_004152337.1	hypothetical protein	-
CSB98_RS00260	18341..18649	-	309	WP_004152336.1	hypothetical protein	-
CSB98_RS00270	19136..19597	-	462	WP_072093212.1	hypothetical protein	-
CSB98_RS00275	19554..19784	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CSB98_RS00280	19781..20197	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
CSB98_RS00285	20271..20981	+	711	WP_004152334.1	ATP-binding protein	-
CSB98_RS00290	21015..21712	+	698	Protein_21	IS1 family transposase	-
CSB98_RS00295	21728..21838	+	111	Protein_22	mercuric transport protein periplasmic component	-
CSB98_RS00300	21874..22296	+	423	WP_001340589.1	organomercurial transporter MerC	-
CSB98_RS00305	22348..24042	+	1695	WP_000105636.1	mercury(II) reductase	-
CSB98_RS00310	24060..24422	+	363	WP_001277456.1	mercury resistance co-regulator MerD	-
CSB98_RS00315	24419..24655	+	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1A / blaOXA-9 / blaKPC-2	-	1..113639	113639
	-	flank	IS/Tn	-	-	13364..14632	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T97504 WP_001044770.1 NZ_CP027158:19781-20197 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T97504 NZ_CP027158:19781-20197 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT97504 WP_001261282.1 NZ_CP027158:19554-19784 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT97504 NZ_CP027158:19554-19784 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3