Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 6442..7085 Replicon plasmid unnamed3
Accession NZ_CP027150
Organism Klebsiella pneumoniae strain AR_0363

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CSC00_RS27205 Protein ID WP_001044770.1
Coordinates 6442..6858 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CSC00_RS27210 Protein ID WP_001261282.1
Coordinates 6855..7085 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC00_RS27170 1984..2220 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
CSC00_RS27175 2217..2579 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
CSC00_RS27180 2597..4291 - 1695 WP_000105636.1 mercury(II) reductase -
CSC00_RS27185 4343..4765 - 423 WP_001340589.1 organomercurial transporter MerC -
CSC00_RS27190 4801..4911 - 111 Protein_6 mercuric transport protein periplasmic component -
CSC00_RS27195 4927..5624 - 698 Protein_7 IS1 family transposase -
CSC00_RS27200 5658..6368 - 711 WP_004152334.1 ATP-binding protein -
CSC00_RS27205 6442..6858 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CSC00_RS27210 6855..7085 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CSC00_RS27215 7042..7503 + 462 WP_072202616.1 hypothetical protein -
CSC00_RS27220 7647..7997 + 351 WP_000493378.1 hypothetical protein -
CSC00_RS27225 8048..8791 + 744 WP_000129823.1 hypothetical protein -
CSC00_RS27230 8788..9564 + 777 WP_000015958.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aac(6')-Ib / aadA2 / cmlA1 / ant(3'')-Ia / aac(3)-IVa / aph(4)-Ia / blaKPC-2 - 1..65684 65684
- inside IScluster/Tn aac(6')-Ib - 4927..15401 10474


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T97501 WP_001044770.1 NZ_CP027150:c6858-6442 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T97501 NZ_CP027150:c6858-6442 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT97501 WP_001261282.1 NZ_CP027150:c7085-6855 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT97501 NZ_CP027150:c7085-6855 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References