Detailed information of TA system TA097482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 60629..61272
• Replicon: plasmid unnamed2
• Accession: NZ_CP027145
• Organism: Enterobacter hormaechei subsp. hoffmannii strain AR_0365

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A155IU92
• Locus tag: CSC02_RS26395
• Protein ID: WP_000754567.1
• Coordinates: 60629..61045 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A155IUD1
• Locus tag: CSC02_RS26400
• Protein ID: WP_023293777.1
• Coordinates: 61042..61272 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC02_RS26360	55713..56294	+	582	WP_008502230.1	TetR/AcrR family transcriptional regulator	-
CSC02_RS26365	56299..56637	+	339	WP_000888080.1	carboxymuconolactone decarboxylase family protein	-
CSC02_RS26370	56667..56996	-	330	WP_008502229.1	thioredoxin family protein	-
CSC02_RS26375	57209..58315	+	1107	WP_006687059.1	alkene reductase	-
CSC02_RS26380	58381..59082	+	702	WP_008502228.1	DsbA family oxidoreductase	-
CSC02_RS26385	59148..59285	+	138	Protein_66	sugar dehydrogenase	-
CSC02_RS26390	59319..60278	+	960	WP_176690897.1	IS5 family transposase	-
CSC02_RS26395	60629..61045	-	417	WP_000754567.1	type II toxin-antitoxin system VapC family toxin	Toxin
CSC02_RS26400	61042..61272	-	231	WP_023293777.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CSC02_RS26410	61935..62141	+	207	WP_063154548.1	hypothetical protein	-
CSC02_RS26415	62206..62910	+	705	WP_063154412.1	hypothetical protein	-
CSC02_RS26420	63196..63489	+	294	WP_063154411.1	hypothetical protein	-
CSC02_RS26425	63519..63848	+	330	WP_063154409.1	hypothetical protein	-
CSC02_RS26430	63896..64288	+	393	WP_162269364.1	hypothetical protein	-
CSC02_RS26435	64301..64636	+	336	WP_063154408.1	hypothetical protein	-
CSC02_RS26440	64633..65415	+	783	WP_063154406.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..101548	101548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15108.61 Da
• Isoelectric Point: 8.5403

>T97482 WP_000754567.1 NZ_CP027145:c61045-60629 [Enterobacter hormaechei subsp. hoffmannii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T97482 NZ_CP027145:c61045-60629 [Enterobacter hormaechei subsp. hoffmannii]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8706.73 Da
• Isoelectric Point: 4.4299

>AT97482 WP_023293777.1 NZ_CP027145:c61272-61042 [Enterobacter hormaechei subsp. hoffmannii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Nucleotide

• Length: 231 bp

>AT97482 NZ_CP027145:c61272-61042 [Enterobacter hormaechei subsp. hoffmannii]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTAG
ACAGGGCCGATCCGGACTTTATGGCGCAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Structures

Toxin

• AlphaFold DB: A0A155IU92

Antitoxin

• AlphaFold DB: A0A155IUD1