Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 194835..195361 Replicon plasmid unnamed1
Accession NZ_CP027144
Organism Enterobacter hormaechei subsp. hoffmannii strain AR_0365

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag CSC02_RS25355 Protein ID WP_000323025.1
Coordinates 194835..195122 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag CSC02_RS25360 Protein ID WP_000534858.1
Coordinates 195122..195361 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC02_RS27145 190405..190581 - 177 WP_001371930.1 hypothetical protein -
CSC02_RS25325 191092..192036 + 945 WP_000778029.1 DUF5417 domain-containing protein -
CSC02_RS25330 192132..192734 + 603 WP_012695474.1 hypothetical protein -
CSC02_RS25335 192794..193144 + 351 WP_000743059.1 hypothetical protein -
CSC02_RS25340 193191..193394 + 204 WP_001015183.1 hypothetical protein -
CSC02_RS25345 193676..193996 + 321 WP_000332796.1 hypothetical protein -
CSC02_RS25350 194605..194763 - 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
CSC02_RS25355 194835..195122 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
CSC02_RS25360 195122..195361 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
CSC02_RS25370 195624..196547 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
CSC02_RS25375 196747..197319 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
CSC02_RS25380 197795..199033 - 1239 WP_000219087.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / sul1 / qacE / ere(A) / aac(6')-IIc / aadA2 / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 - 1..328871 328871


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T97481 WP_000323025.1 NZ_CP027144:c195122-194835 [Enterobacter hormaechei subsp. hoffmannii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T97481 NZ_CP027144:c195122-194835 [Enterobacter hormaechei subsp. hoffmannii]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT97481 WP_000534858.1 NZ_CP027144:c195361-195122 [Enterobacter hormaechei subsp. hoffmannii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT97481 NZ_CP027144:c195361-195122 [Enterobacter hormaechei subsp. hoffmannii]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References