Detailed information of TA system TA097478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3833975..3834591
• Replicon: chromosome
• Accession: NZ_CP027142
• Organism: Enterobacter hormaechei subsp. hoffmannii strain AR_0365

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CSC02_RS19025
• Protein ID: WP_080346796.1
• Coordinates: 3833975..3834346 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A9E7BIL4
• Locus tag: CSC02_RS19030
• Protein ID: WP_023298920.1
• Coordinates: 3834349..3834591 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC02_RS19010	3831475..3832377	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
CSC02_RS19015	3832374..3833009	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
CSC02_RS19020	3833006..3833935	+	930	WP_023305104.1	formate dehydrogenase accessory protein FdhE	-
CSC02_RS19025	3833975..3834346	-	372	WP_080346796.1	type II toxin-antitoxin system VapC family toxin	Toxin
CSC02_RS19030	3834349..3834591	-	243	WP_023298920.1	ribbon-helix-helix protein, CopG family	Antitoxin
CSC02_RS19035	3834791..3835711	+	921	WP_023305106.1	alpha/beta hydrolase	-
CSC02_RS19040	3835720..3836661	-	942	WP_023305107.1	fatty acid biosynthesis protein FabY	-
CSC02_RS19045	3836706..3837143	-	438	WP_023305108.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CSC02_RS19050	3837140..3838021	-	882	WP_023298923.1	virulence factor BrkB family protein	-
CSC02_RS19055	3838015..3838614	-	600	WP_023305109.1	glucose-1-phosphatase	-
CSC02_RS19060	3838733..3839533	-	801	WP_023305110.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-114)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13668.73 Da
• Isoelectric Point: 6.2151

>T97478 WP_080346796.1 NZ_CP027142:c3834346-3833975 [Enterobacter hormaechei subsp. hoffmannii]
MEHMAVFDTNILIDLFNNHVEAADAIDRTASHRAISLITWMEVMVGARRHGQEAKTAAVMEAFEIIDITREIAERSVLLR
EQHGMKLPDAIILATAQSRSCPLISRNTKDFSGIAGVVSPYHL

Nucleotide

• Length: 372 bp

>T97478 NZ_CP027142:c3834346-3833975 [Enterobacter hormaechei subsp. hoffmannii]
ATGGAGCACATGGCTGTGTTTGACACCAATATCCTCATCGATCTTTTTAATAACCACGTTGAGGCTGCGGATGCGATCGA
TCGCACAGCTTCGCATCGTGCCATTAGCCTCATCACATGGATGGAAGTAATGGTGGGCGCTCGCAGGCACGGTCAAGAAG
CAAAAACGGCAGCCGTGATGGAGGCCTTTGAAATTATTGATATAACAAGGGAAATTGCTGAAAGAAGCGTATTACTTCGC
GAACAGCACGGGATGAAGCTTCCTGACGCCATTATTCTGGCTACTGCTCAGAGCAGAAGCTGTCCGTTAATTTCGCGCAA
TACGAAAGACTTTTCGGGTATTGCTGGGGTCGTTTCACCTTACCACCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9467.74 Da
• Isoelectric Point: 4.3775

>AT97478 WP_023298920.1 NZ_CP027142:c3834591-3834349 [Enterobacter hormaechei subsp. hoffmannii]
MSLLADLTMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIELYLDQQNSSVIREALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT97478 NZ_CP027142:c3834591-3834349 [Enterobacter hormaechei subsp. hoffmannii]
ATGAGTCTTTTGGCGGACCTTACTATGGGCAGAATACTGATCGATTTGTCTGATGACGTGATTCAACGACTGGATAACCT
GAAGCAGTTGCGCAACCGGCCGCGCGCCGAACTGCTGCGGGAAGCCATAGAACTGTATCTCGACCAGCAGAATTCATCGG
TTATCCGCGAAGCGCTTGGCCTGTGGGGAAATCAGCAGGAAGATGGGCTTGAGTACGAACGCAAGCTGCGTGAGGAGTGG
TAA