Detailed information of TA system TA097467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1448046..1448785
• Replicon: chromosome
• Accession: NZ_CP027142
• Organism: Enterobacter hormaechei subsp. hoffmannii strain AR_0365

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: CSC02_RS07300
• Protein ID: WP_032624244.1
• Coordinates: 1448046..1448531 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: CSC02_RS07305
• Protein ID: WP_003857131.1
• Coordinates: 1448519..1448785 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC02_RS07285	1446733..1447014	+	282	Protein_1393	DUF4225 domain-containing protein	-
CSC02_RS07290	1446989..1447318	-	330	WP_023306210.1	hypothetical protein	-
CSC02_RS07295	1447451..1448020	+	570	WP_023306211.1	hypothetical protein	-
CSC02_RS07300	1448046..1448531	-	486	WP_032624244.1	GNAT family N-acetyltransferase	Toxin
CSC02_RS07305	1448519..1448785	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
CSC02_RS07310	1448849..1449778	-	930	WP_023306213.1	LysR family transcriptional regulator	-
CSC02_RS07315	1449908..1451296	+	1389	WP_105322750.1	MFS transporter	-
CSC02_RS07320	1451275..1451832	-	558	WP_023300131.1	OmpH family outer membrane protein	-
CSC02_RS07325	1451953..1453089	-	1137	WP_105322751.1	type 1 fimbrial protein	-
CSC02_RS07330	1453113..1453637	-	525	WP_087878309.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17531.27 Da
• Isoelectric Point: 10.1788

>T97467 WP_032624244.1 NZ_CP027142:c1448531-1448046 [Enterobacter hormaechei subsp. hoffmannii]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEKAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T97467 NZ_CP027142:c1448531-1448046 [Enterobacter hormaechei subsp. hoffmannii]
GTGGGACGTGTAACCGCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTACTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AAAAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT97467 WP_003857131.1 NZ_CP027142:c1448785-1448519 [Enterobacter hormaechei subsp. hoffmannii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT97467 NZ_CP027142:c1448785-1448519 [Enterobacter hormaechei subsp. hoffmannii]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTACA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9