Detailed information of TA system TA097444

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 1095137..1095816
• Replicon: chromosome
• Accession: NZ_CP027140
• Organism: Escherichia coli strain AR_0369

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: CSC06_RS07815
• Protein ID: WP_000854680.1
• Coordinates: 1095475..1095816 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: CSC06_RS07810
• Protein ID: WP_000070396.1
• Coordinates: 1095137..1095454 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC06_RS07765	1090575..1091396	+	822	WP_032200808.1	DUF945 domain-containing protein	-
CSC06_RS07770	1091613..1092314	+	702	WP_001581462.1	WYL domain-containing protein	-
CSC06_RS07775	1092355..1092591	+	237	WP_001144031.1	hypothetical protein	-
CSC06_RS07780	1092591..1093034	+	444	WP_032199444.1	hypothetical protein	-
CSC06_RS07785	1093057..1093524	+	468	WP_032200803.1	hypothetical protein	-
CSC06_RS07790	1093601..1093840	+	240	WP_000194654.1	DUF905 domain-containing protein	-
CSC06_RS07795	1093938..1094396	+	459	WP_032177818.1	antirestriction protein	-
CSC06_RS07800	1094412..1094888	+	477	WP_000811693.1	RadC family protein	-
CSC06_RS07805	1094897..1095118	+	222	WP_000691994.1	DUF987 domain-containing protein	-
CSC06_RS07810	1095137..1095454	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
CSC06_RS07815	1095475..1095816	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
CSC06_RS07825	1096388..1097641	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
CSC06_RS07830	1097653..1098756	-	1104	WP_001285288.1	glutamate 5-kinase	-
CSC06_RS07835	1099044..1100099	+	1056	WP_000749881.1	phosphoporin PhoE	-
CSC06_RS07840	1100138..1100539	-	402	WP_000174677.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / tssM / tssA / hcp1/tssD1	1072495..1129686	57191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T97444 WP_000854680.1 NZ_CP027140:1095475-1095816 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T97444 NZ_CP027140:1095475-1095816 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT97444 WP_000070396.1 NZ_CP027140:1095137-1095454 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT97444 NZ_CP027140:1095137-1095454 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7