Detailed information of TA system TA097440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 30069..30591
• Replicon: plasmid unnamed2
• Accession: NZ_CP027138
• Organism: Escherichia coli strain AR_0369

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: CSC06_RS01475
• Protein ID: WP_000220560.1
• Coordinates: 30310..30591 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: CSC06_RS01470
• Protein ID: WP_000121743.1
• Coordinates: 30069..30320 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC06_RS01435	25147..25809	+	663	WP_050871575.1	peptidyl-arginine deiminase	-
CSC06_RS01440	25909..26190	+	282	WP_001185482.1	hypothetical protein	-
CSC06_RS01445	26215..27192	+	978	WP_001579336.1	hypothetical protein	-
CSC06_RS01450	27189..27614	-	426	WP_015058974.1	TaxD	-
CSC06_RS01455	27682..28128	-	447	WP_001074386.1	hypothetical protein	-
CSC06_RS01460	28153..29007	-	855	WP_001239324.1	replication initiation protein	-
CSC06_RS01470	30069..30320	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
CSC06_RS01475	30310..30591	+	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSC06_RS01480	30737..31060	+	324	WP_001181903.1	hypothetical protein	-
CSC06_RS01485	31105..31350	+	246	WP_000356546.1	hypothetical protein	-
CSC06_RS01490	31340..31555	+	216	WP_001180116.1	hypothetical protein	-
CSC06_RS01495	31648..31977	+	330	WP_000866648.1	hypothetical protein	-
CSC06_RS01500	32020..32199	+	180	WP_000439078.1	hypothetical protein	-
CSC06_RS26350	32269..32418	+	150	WP_000003880.1	hypothetical protein	-
CSC06_RS01510	32431..32703	+	273	WP_000160399.1	hypothetical protein	-
CSC06_RS26540	32960..33034	+	75	Protein_43	DNA distortion polypeptide 3	-
CSC06_RS01520	33029..33574	+	546	WP_105394090.1	DNA distortion polypeptide 1	-
CSC06_RS01525	33577..34743	+	1167	WP_105394073.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / dfrA8 / sul3 / ant(3'')-Ia / catA3	-	1..90735	90735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T97440 WP_000220560.1 NZ_CP027138:30310-30591 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T97440 NZ_CP027138:30310-30591 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT97440 WP_000121743.1 NZ_CP027138:30069-30320 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT97440 NZ_CP027138:30069-30320 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301