Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 68242..68885 Replicon plasmid unnamed3
Accession NZ_CP027135
Organism Escherichia coli strain AR_0369

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag CSC06_RS00455 Protein ID WP_001034044.1
Coordinates 68469..68885 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag CSC06_RS00450 Protein ID WP_001261286.1
Coordinates 68242..68472 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC06_RS00420 63492..63908 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
CSC06_RS00425 63901..64881 - 981 WP_000688514.1 plasmid segregation protein ParM -
CSC06_RS00430 65295..65603 - 309 WP_000030199.1 hypothetical protein -
CSC06_RS00435 65690..66334 - 645 WP_001144036.1 ParA family protein -
CSC06_RS00440 66514..67293 - 780 WP_001164198.1 site-specific integrase -
CSC06_RS00445 67295..67708 - 414 WP_000465034.1 hypothetical protein -
CSC06_RS00450 68242..68472 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CSC06_RS00455 68469..68885 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
CSC06_RS00460 68960..70525 + 1566 WP_001128474.1 AAA family ATPase -
CSC06_RS00465 70510..71532 + 1023 WP_000361402.1 helicase UvrD -
CSC06_RS00475 71786..72472 - 687 Protein_81 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / blaTEM-1D iutA / iucD / iucC / iucB / iucA 1..151026 151026
- flank IS/Tn - - 71786..72133 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T97439 WP_001034044.1 NZ_CP027135:68469-68885 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T97439 NZ_CP027135:68469-68885 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT97439 WP_001261286.1 NZ_CP027135:68242-68472 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT97439 NZ_CP027135:68242-68472 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References