Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4178675..4179329 Replicon chromosome
Accession NZ_CP027134
Organism Escherichia coli strain AR_0372

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CSC09_RS23285 Protein ID WP_000244781.1
Coordinates 4178675..4179082 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CSC09_RS23290 Protein ID WP_000354046.1
Coordinates 4179063..4179329 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC09_RS23265 4174632..4176365 - 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -
CSC09_RS23270 4176371..4177081 - 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CSC09_RS23275 4177106..4178002 - 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
CSC09_RS23280 4178114..4178635 + 522 WP_001055874.1 flavodoxin FldB -
CSC09_RS23285 4178675..4179082 - 408 WP_000244781.1 protein YgfX Toxin
CSC09_RS23290 4179063..4179329 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CSC09_RS23295 4179572..4180552 + 981 WP_000886087.1 tRNA-modifying protein YgfZ -
CSC09_RS23300 4180629..4181288 - 660 WP_000250274.1 hemolysin III family protein -
CSC09_RS23305 4181452..4181763 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
CSC09_RS23310 4181808..4183241 + 1434 WP_001394742.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T97432 WP_000244781.1 NZ_CP027134:c4179082-4178675 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T97432 NZ_CP027134:c4179082-4178675 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGTG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT97432 WP_000354046.1 NZ_CP027134:c4179329-4179063 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT97432 NZ_CP027134:c4179329-4179063 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References