Detailed information of TA system TA097420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2589828..2590354
• Replicon: chromosome
• Accession: NZ_CP027134
• Organism: Escherichia coli strain AR_0372

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: CSC09_RS15075
• Protein ID: WP_000323025.1
• Coordinates: 2589828..2590115 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: CSC09_RS15080
• Protein ID: WP_000534858.1
• Coordinates: 2590115..2590354 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC09_RS15035	2584852..2585067	-	216	WP_000839590.1	phage lysis protein EssD	-
CSC09_RS29500	2585287..2585457	+	171	WP_211180497.1	putative zinc-binding protein YnfU	-
CSC09_RS15040	2585821..2586036	-	216	WP_000066484.1	cold shock-like protein CspB	-
CSC09_RS15045	2586337..2586549	+	213	WP_000087756.1	cold shock-like protein CspF	-
CSC09_RS28960	2586604..2586693	+	90	WP_120795389.1	hypothetical protein	-
CSC09_RS15050	2586971..2587723	-	753	WP_001047135.1	antitermination protein	-
CSC09_RS15055	2587737..2588786	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
CSC09_RS15060	2588788..2589066	-	279	WP_012304870.1	hypothetical protein	-
CSC09_RS15065	2589133..2589384	-	252	WP_000980994.1	hypothetical protein	-
CSC09_RS15070	2589601..2589756	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
CSC09_RS15075	2589828..2590115	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
CSC09_RS15080	2590115..2590354	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
CSC09_RS15085	2590379..2590684	+	306	WP_001326990.1	hypothetical protein	-
CSC09_RS15090	2590887..2591219	+	333	WP_001301033.1	protein FlxA	-
CSC09_RS15100	2591656..2592969	-	1314	WP_000589005.1	ISNCY family transposase	-
CSC09_RS15105	2593453..2594481	-	1029	WP_001345551.1	DUF4868 domain-containing protein	-
CSC09_RS15110	2594478..2595092	-	615	WP_001265627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2556935..2608778	51843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T97420 WP_000323025.1 NZ_CP027134:c2590115-2589828 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T97420 NZ_CP027134:c2590115-2589828 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT97420 WP_000534858.1 NZ_CP027134:c2590354-2590115 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT97420 NZ_CP027134:c2590354-2590115 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8