Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1292198..1292816 Replicon chromosome
Accession NZ_CP027134
Organism Escherichia coli strain AR_0372

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CSC09_RS08405 Protein ID WP_001291435.1
Coordinates 1292198..1292416 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CSC09_RS08410 Protein ID WP_000344800.1
Coordinates 1292442..1292816 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC09_RS08370 1287487..1288059 + 573 WP_000779831.1 YbaY family lipoprotein -
CSC09_RS08375 1288090..1288401 - 312 WP_000409911.1 MGMT family protein -
CSC09_RS08385 1288780..1289133 + 354 WP_000878140.1 DUF1428 domain-containing protein -
CSC09_RS08390 1289175..1290725 - 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CSC09_RS08395 1290889..1291359 - 471 WP_001490888.1 YlaC family protein -
CSC09_RS08400 1291475..1292026 - 552 WP_001522034.1 maltose O-acetyltransferase -
CSC09_RS08405 1292198..1292416 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CSC09_RS08410 1292442..1292816 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CSC09_RS08415 1293362..1296511 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
CSC09_RS08420 1296534..1297727 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T97409 WP_001291435.1 NZ_CP027134:c1292416-1292198 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T97409 NZ_CP027134:c1292416-1292198 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT97409 WP_000344800.1 NZ_CP027134:c1292816-1292442 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT97409 NZ_CP027134:c1292816-1292442 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References