Detailed information of TA system TA097408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 1083424..1085113
• Replicon: chromosome
• Accession: NZ_CP027134
• Organism: Escherichia coli strain AR_0372

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A829L165
• Locus tag: CSC09_RS07335
• Protein ID: WP_001521912.1
• Coordinates: 1084193..1085113 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: CSC09_RS07330
• Protein ID: WP_001521911.1
• Coordinates: 1083424..1084206 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC09_RS29195	1078733..1078891	-	159	Protein_986	integrase	-
CSC09_RS29200	1078983..1079085	-	103	Protein_987	helix-turn-helix domain-containing protein	-
CSC09_RS07320	1079082..1079552	-	471	WP_000201185.1	hypothetical protein	-
CSC09_RS28650	1079598..1079738	-	141	WP_001308408.1	ash family protein	-
CSC09_RS07325	1079899..1083108	-	3210	WP_001521910.1	RNA-directed DNA polymerase	-
CSC09_RS07330	1083424..1084206	+	783	WP_001521911.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
CSC09_RS07335	1084193..1085113	+	921	WP_001521912.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CSC09_RS07345	1085547..1086431	-	885	WP_001119037.1	LysR family transcriptional regulator	-
CSC09_RS07350	1086696..1087754	+	1059	WP_122452191.1	alpha/beta hydrolase	-
CSC09_RS07355	1087896..1089023	+	1128	WP_001521913.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34601.04 Da
• Isoelectric Point: 7.2649

>T97408 WP_001521912.1 NZ_CP027134:1084193-1085113 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T97408 NZ_CP027134:1084193-1085113 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCCGGCAGGTCAAACTGTTGATGGCCGCTCTGCCGTATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACCGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACTGATCTGGCGGCTATCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCAGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATTGCCGAGTTGCTGGCCCCAAACTGGGATACAGAGCGGATCACGACGCTGTATGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTGCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGTAATCTGGCGGTGCTGAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGCGTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29168.46 Da
• Isoelectric Point: 8.4458

>AT97408 WP_001521911.1 NZ_CP027134:1083424-1084206 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELVAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQFIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT97408 NZ_CP027134:1083424-1084206 [Escherichia coli]
TTGCCAAACTATCTAAACTGGCTGATGCAGCACACGCTACCCGGCCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTGAAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTATACTGCCGGACGG
GTCGTGAACCGGATTGGGTGGATGCGGTTTACTGCCTGCAATCGCAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGTCATGCGCACTATACCGAACAGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTTCTGCCTTTGAACAATCCGCAACATTTGTCACGCTGTATACGTCAGGCCTTACCGCTGATGTAA
CGCCATTTACTACGGAACTGAAAATTGGTGACCGGCAGTTAGCGGGCAGTGTCCCGGAACTGGTCGCGTATGAGATCGCT
TGTGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGATGAATCTTCTCAGCCCGCGTAAATT
GCAGGCGTTACTCTGCGCCAGTCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTTTATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A829L165