Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 140433..140958 Replicon plasmid unnamed1
Accession NZ_CP027130
Organism Escherichia coli strain AR_0372

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CSC09_RS01020 Protein ID WP_001159871.1
Coordinates 140433..140738 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag CSC09_RS01025 Protein ID WP_000813630.1
Coordinates 140740..140958 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC09_RS01005 136395..137561 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
CSC09_RS01010 138149..138904 - 756 WP_000852146.1 replication initiation protein RepE -
CSC09_RS01015 139626..140432 - 807 WP_000016970.1 site-specific integrase -
CSC09_RS01020 140433..140738 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CSC09_RS01025 140740..140958 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CSC09_RS01035 141518..141748 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CSC09_RS01040 141745..142161 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
CSC09_RS01045 142236..143801 + 1566 WP_001128474.1 AAA family ATPase -
CSC09_RS01050 143786..144808 + 1023 WP_000361402.1 helicase UvrD -
CSC09_RS01060 145062..145748 - 687 Protein_178 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD iutA / iucD / iucC / iucB / iucA 1..170292 170292
- flank IS/Tn - - 145062..145409 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T97397 WP_001159871.1 NZ_CP027130:c140738-140433 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T97397 NZ_CP027130:c140738-140433 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT97397 WP_000813630.1 NZ_CP027130:c140958-140740 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT97397 NZ_CP027130:c140958-140740 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References