Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 23163..23688 Replicon plasmid unnamed2
Accession NZ_CP027127
Organism Escherichia coli strain AR_0374

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CSC11_RS25535 Protein ID WP_001159871.1
Coordinates 23163..23468 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag CSC11_RS25540 Protein ID WP_000813630.1
Coordinates 23470..23688 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC11_RS25510 19334..19750 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
CSC11_RS25515 19743..20723 - 981 WP_000688514.1 plasmid segregation protein ParM -
CSC11_RS25520 21137..21445 - 309 WP_000030199.1 hypothetical protein -
CSC11_RS25525 21532..22176 - 645 WP_001144036.1 ParA family protein -
CSC11_RS25530 22356..23162 - 807 WP_000016969.1 site-specific integrase -
CSC11_RS25535 23163..23468 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CSC11_RS25540 23470..23688 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CSC11_RS25550 24246..25172 + 927 WP_001290416.1 hypothetical protein -
CSC11_RS25555 25217..25471 + 255 WP_000678527.1 hypothetical protein -
CSC11_RS25560 25970..26314 + 345 WP_000793307.1 hypothetical protein -
CSC11_RS25565 26490..27491 - 1002 WP_000774875.1 DUF4238 domain-containing protein -
CSC11_RS25570 27843..28097 + 255 WP_000343103.1 hypothetical protein -
CSC11_RS25575 28097..28687 + 591 WP_000194550.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCMY-2 - 1..98987 98987


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T97383 WP_001159871.1 NZ_CP027127:c23468-23163 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T97383 NZ_CP027127:c23468-23163 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT97383 WP_000813630.1 NZ_CP027127:c23688-23470 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT97383 NZ_CP027127:c23688-23470 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References