Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4431049..4431674 Replicon chromosome
Accession NZ_CP027126
Organism Escherichia coli strain AR_0374

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag CSC11_RS23195 Protein ID WP_000911329.1
Coordinates 4431276..4431674 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag CSC11_RS23190 Protein ID WP_000450524.1
Coordinates 4431049..4431276 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC11_RS23165 4426851..4427321 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
CSC11_RS23170 4427321..4427893 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
CSC11_RS23175 4428039..4428917 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
CSC11_RS23180 4428934..4429968 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
CSC11_RS23185 4430181..4430894 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
CSC11_RS23190 4431049..4431276 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
CSC11_RS23195 4431276..4431674 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
CSC11_RS23200 4431821..4432684 + 864 WP_001267498.1 neutral zinc metallopeptidase -
CSC11_RS23205 4432699..4434714 + 2016 WP_000829295.1 tRNA cytosine(34) acetyltransferase TmcA -
CSC11_RS23210 4434788..4435486 + 699 WP_000679823.1 esterase -
CSC11_RS23215 4435596..4435796 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T97381 WP_000911329.1 NZ_CP027126:4431276-4431674 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T97381 NZ_CP027126:4431276-4431674 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT97381 WP_000450524.1 NZ_CP027126:4431049-4431276 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT97381 NZ_CP027126:4431049-4431276 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References