Detailed information of TA system TA097371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1735389..1736226
• Replicon: chromosome
• Accession: NZ_CP027126
• Organism: Escherichia coli strain AR_0374

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: CSC11_RS09320
• Protein ID: WP_000227788.1
• Coordinates: 1735684..1736226 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: CSC11_RS09315
• Protein ID: WP_001297137.1
• Coordinates: 1735389..1735700 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC11_RS09290	1730409..1731356	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
CSC11_RS09295	1731378..1733369	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
CSC11_RS09300	1733359..1733973	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
CSC11_RS09305	1733973..1734302	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CSC11_RS09310	1734314..1735204	+	891	WP_000971332.1	heme o synthase	-
CSC11_RS09315	1735389..1735700	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
CSC11_RS09320	1735684..1736226	+	543	WP_000227788.1	GNAT family N-acetyltransferase	Toxin
CSC11_RS09325	1736282..1737217	-	936	WP_001297127.1	sel1 repeat family protein	-
CSC11_RS09330	1737625..1738989	+	1365	WP_001000978.1	MFS transporter	-
CSC11_RS09335	1739117..1739608	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
CSC11_RS09340	1739776..1740687	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (79-160)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19835.05 Da
• Isoelectric Point: 8.3395

>T97371 WP_000227788.1 NZ_CP027126:1735684-1736226 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFSQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T97371 NZ_CP027126:1735684-1736226 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTAGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT97371 WP_001297137.1 NZ_CP027126:1735389-1735700 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT97371 NZ_CP027126:1735389-1735700 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
TGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3