Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 677416..678054 Replicon chromosome
Accession NZ_CP027126
Organism Escherichia coli strain AR_0374

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CSC11_RS03915 Protein ID WP_000813794.1
Coordinates 677878..678054 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CSC11_RS03910 Protein ID WP_001270285.1
Coordinates 677416..677832 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC11_RS03890 672568..673509 - 942 WP_001251313.1 ABC transporter permease -
CSC11_RS03895 673510..674523 - 1014 WP_000220411.1 ABC transporter ATP-binding protein -
CSC11_RS03900 674541..675686 - 1146 WP_000034363.1 ABC transporter substrate-binding protein -
CSC11_RS03905 675931..677337 - 1407 WP_000760590.1 PLP-dependent aminotransferase family protein -
CSC11_RS03910 677416..677832 - 417 WP_001270285.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CSC11_RS03915 677878..678054 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CSC11_RS03920 678276..678506 + 231 WP_000494244.1 YncJ family protein -
CSC11_RS03925 678598..680559 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CSC11_RS03930 680632..681168 - 537 WP_000429153.1 DNA-binding transcriptional regulator SutR -
CSC11_RS03935 681221..682435 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 682475..683623 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(88-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T97369 WP_000813794.1 NZ_CP027126:c678054-677878 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T97369 NZ_CP027126:c678054-677878 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15247.61 Da        Isoelectric Point: 4.6115

>AT97369 WP_001270285.1 NZ_CP027126:c677832-677416 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT97369 NZ_CP027126:c677832-677416 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References