Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 28556..29199 Replicon plasmid unnamed1
Accession NZ_CP027119
Organism Escherichia coli strain 26561

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag CYQ10_RS24470 Protein ID WP_001034046.1
Coordinates 28556..28972 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag CYQ10_RS24475 Protein ID WP_001261278.1
Coordinates 28969..29199 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYQ10_RS24455 23699..24115 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
CYQ10_RS24460 24112..24341 - 230 Protein_23 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CYQ10_RS24465 24722..28512 + 3791 Protein_24 pcar -
CYQ10_RS24470 28556..28972 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
CYQ10_RS24475 28969..29199 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CYQ10_RS24480 29464..29964 + 501 WP_000528931.1 hypothetical protein -
CYQ10_RS24485 29977..30750 + 774 WP_000905949.1 hypothetical protein -
CYQ10_RS24490 30917..32049 + 1133 Protein_29 DUF3800 domain-containing protein -
CYQ10_RS24495 32083..32595 - 513 WP_000151784.1 hypothetical protein -
CYQ10_RS24500 33162..33380 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
CYQ10_RS24505 33382..33686 + 305 Protein_32 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / tet(B) iucA / iucB / iucB / iucC / iucD / iutA 1..86731 86731


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T97355 WP_001034046.1 NZ_CP027119:c28972-28556 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T97355 NZ_CP027119:c28972-28556 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT97355 WP_001261278.1 NZ_CP027119:c29199-28969 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT97355 NZ_CP027119:c29199-28969 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References