Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3932408..3932984 Replicon chromosome
Accession NZ_CP027118
Organism Escherichia coli strain 26561

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag CYQ10_RS20250 Protein ID WP_001297643.1
Coordinates 3932697..3932984 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag CYQ10_RS20245 Protein ID WP_000063156.1
Coordinates 3932408..3932710 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYQ10_RS20230 3929004..3931154 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
CYQ10_RS20235 3931204..3931407 + 204 WP_000467859.1 YbdD/YjiX family protein -
CYQ10_RS20240 3931418..3932374 + 957 WP_001297640.1 GTPase -
CYQ10_RS20245 3932408..3932710 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
CYQ10_RS20250 3932697..3932984 - 288 WP_001297643.1 BrnT family toxin Toxin
CYQ10_RS20255 3933183..3933880 - 698 Protein_3694 IS1 family transposase -
CYQ10_RS20260 3933937..3934032 + 96 Protein_3695 dienelactone hydrolase family protein -
CYQ10_RS20265 3934178..3935410 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
CYQ10_RS20270 3935451..3936731 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - cheD 3890983..3933686 42703


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T97353 WP_001297643.1 NZ_CP027118:c3932984-3932697 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T97353 NZ_CP027118:c3932984-3932697 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT97353 WP_000063156.1 NZ_CP027118:c3932710-3932408 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT97353 NZ_CP027118:c3932710-3932408 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References