Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 525804..526440 Replicon chromosome
Accession NZ_CP027116
Organism Bacillus pumilus strain 145

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag C5695_RS02520 Protein ID WP_003214169.1
Coordinates 526090..526440 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag C5695_RS02515 Protein ID WP_003214273.1
Coordinates 525804..526085 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5695_RS02495 521951..522556 - 606 WP_117728881.1 rhomboid family intramembrane serine protease -
C5695_RS02500 522651..523016 + 366 WP_117728883.1 holo-ACP synthase -
C5695_RS02505 523177..524193 + 1017 WP_117728885.1 outer membrane lipoprotein carrier protein LolA -
C5695_RS02510 524329..525510 + 1182 WP_117728887.1 alanine racemase -
C5695_RS02515 525804..526085 + 282 WP_003214273.1 hypothetical protein Antitoxin
C5695_RS02520 526090..526440 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
C5695_RS02525 526557..527387 + 831 WP_117728889.1 RsbT co-antagonist protein RsbRA -
C5695_RS02530 527392..527760 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
C5695_RS02535 527763..528164 + 402 WP_117728892.1 anti-sigma regulatory factor -
C5695_RS02540 528175..529182 + 1008 WP_117728894.1 PP2C family protein-serine/threonine phosphatase -
C5695_RS02545 529242..529571 + 330 WP_117728896.1 anti-sigma factor antagonist -
C5695_RS02550 529568..530056 + 489 WP_117728898.1 anti-sigma B factor RsbW -
C5695_RS02555 530022..530810 + 789 WP_012009000.1 RNA polymerase sigma factor SigB -
C5695_RS02560 530810..531409 + 600 WP_117728900.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T97332 WP_003214169.1 NZ_CP027116:526090-526440 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T97332 NZ_CP027116:526090-526440 [Bacillus pumilus]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGCACAATCGATAAACAAAGATTGACAGATAAAATTACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT97332 WP_003214273.1 NZ_CP027116:525804-526085 [Bacillus pumilus]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT97332 NZ_CP027116:525804-526085 [Bacillus pumilus]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTACCGGAGGCTTTGATGTCTGAAATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References