Detailed information of TA system TA097328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4874702..4875318
• Replicon: chromosome
• Accession: NZ_CP027111
• Organism: Enterobacter hormaechei strain BW

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C5I89_RS23215
• Protein ID: WP_017382676.1
• Coordinates: 4874702..4875073 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: C5I89_RS23220
• Protein ID: WP_015569912.1
• Coordinates: 4875076..4875318 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5I89_RS23200	4872202..4873104	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
C5I89_RS23205	4873101..4873736	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
C5I89_RS23210	4873733..4874662	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
C5I89_RS23215	4874702..4875073	-	372	WP_017382676.1	type II toxin-antitoxin system VapC family toxin	Toxin
C5I89_RS23220	4875076..4875318	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
C5I89_RS23225	4875517..4876437	+	921	WP_017382675.1	alpha/beta hydrolase	-
C5I89_RS23230	4876446..4877387	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
C5I89_RS23235	4877432..4877869	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C5I89_RS23240	4877866..4878747	-	882	WP_003861949.1	virulence factor BrkB family protein	-
C5I89_RS23245	4878741..4879340	-	600	WP_003861947.1	glucose-1-phosphatase	-
C5I89_RS23250	4879459..4880259	-	801	WP_017382673.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13737.88 Da
• Isoelectric Point: 6.4882

>T97328 WP_017382676.1 NZ_CP027111:c4875073-4874702 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T97328 NZ_CP027111:c4875073-4874702 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTATTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT97328 WP_015569912.1 NZ_CP027111:c4875318-4875076 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT97328 NZ_CP027111:c4875318-4875076 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3