Detailed information of TA system TA097308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2276426..2277045
• Replicon: chromosome
• Accession: NZ_CP027107
• Organism: Cronobacter sakazakii strain CS-931

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A2S9UIC9
• Locus tag: CsakCS931_RS10905
• Protein ID: WP_004386909.1
• Coordinates: 2276827..2277045 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CsakCS931_RS10900
• Protein ID: WP_097573307.1
• Coordinates: 2276426..2276800 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CsakCS931_RS10890	2271587..2272792	+	1206	WP_007848022.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
CsakCS931_RS10895	2272814..2275963	+	3150	WP_007848020.1	multidrug efflux RND transporter permease subunit	-
CsakCS931_RS10900	2276426..2276800	+	375	WP_097573307.1	Hha toxicity modulator TomB	Antitoxin
CsakCS931_RS10905	2276827..2277045	+	219	WP_004386909.1	hemolysin expression modulator Hha	Toxin
CsakCS931_RS10910	2277252..2277803	+	552	WP_097573305.1	maltose O-acetyltransferase	-
CsakCS931_RS10915	2277923..2278432	+	510	WP_007891920.1	YlaC family protein	-
CsakCS931_RS10920	2278461..2278601	-	141	WP_007891919.1	type B 50S ribosomal protein L36	-
CsakCS931_RS10925	2278605..2278865	-	261	WP_004386912.1	type B 50S ribosomal protein L31	-
CsakCS931_RS10930	2279005..2280906	-	1902	WP_097573303.1	methyl-accepting chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8598.02 Da
• Isoelectric Point: 9.4828

>T97308 WP_004386909.1 NZ_CP027107:2276827-2277045 [Cronobacter sakazakii]
MTTKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Nucleotide

• Length: 219 bp

>T97308 NZ_CP027107:2276827-2277045 [Cronobacter sakazakii]
ATGACGACCAAACCATTAACCAAAACCGATTATTTAATGCGACTGAGACGTTGTCAGACAATTGACACGCTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTATCCGACAACGAACTGGCGGTATTTTATTCAGCGGCGGATCATCGTCTGGCTGAAC
TGACGATGAATAAGCTCTACGATAAAATTCCGACCGCCGTGTGGAAGTTCGTTCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14342.11 Da
• Isoelectric Point: 5.1389

>AT97308 WP_097573307.1 NZ_CP027107:2276426-2276800 [Cronobacter sakazakii]
MDEYSPKRHDIAQLKFLCESLYHDCLATLGENQHGWVNDPTSAVNLQLNDLIEHIASFGLNYKIKHKDDAALIEQLDEYL
DDTFMLFSNYGINAQDLQKWRKSGNRLFRCFVNASKENPVSLSF

Nucleotide

• Length: 375 bp

>AT97308 NZ_CP027107:2276426-2276800 [Cronobacter sakazakii]
ATGGACGAATACTCACCAAAACGGCATGATATAGCGCAGCTTAAATTTCTTTGCGAAAGCCTGTATCATGATTGCCTTGC
CACGCTCGGCGAAAACCAGCATGGCTGGGTTAACGACCCCACTTCCGCCGTTAACCTGCAACTCAACGACTTGATTGAGC
ATATTGCCAGCTTCGGCCTGAATTATAAAATTAAGCATAAAGATGACGCAGCTCTCATTGAGCAATTAGATGAATATCTG
GATGATACCTTTATGCTGTTCAGCAATTACGGTATCAATGCGCAAGATTTACAAAAATGGCGCAAGTCCGGAAACAGGCT
TTTCCGGTGTTTTGTAAACGCCAGCAAAGAAAACCCTGTAAGCTTGTCTTTTTAA

Structures

Toxin

• AlphaFold DB: A0A2S9UIC9