Detailed information of TA system TA097287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 42174..42699
• Replicon: plasmid pRM14721
• Accession: NZ_CP027106
• Organism: Escherichia coli strain RM14721

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: T2HS38
• Locus tag: C5097_RS24545
• Protein ID: WP_001159861.1
• Coordinates: 42394..42699 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: T2HUI2
• Locus tag: C5097_RS24540
• Protein ID: WP_000813631.1
• Coordinates: 42174..42392 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5097_RS24505	37707..37874	+	168	WP_106668531.1	transcriptional regulator	-
C5097_RS24510	37995..38735	+	741	WP_001066925.1	tyrosine-type recombinase/integrase	-
C5097_RS24515	38978..39955	-	978	WP_106668532.1	RepB family plasmid replication initiator protein	-
C5097_RS24525	40733..41323	-	591	WP_106668533.1	hypothetical protein	-
C5097_RS24530	41323..41586	-	264	WP_106668534.1	hypothetical protein	-
C5097_RS24540	42174..42392	+	219	WP_000813631.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
C5097_RS24545	42394..42699	+	306	WP_001159861.1	type II toxin-antitoxin system toxin CcdB	Toxin
C5097_RS24550	42700..43254	+	555	Protein_44	tyrosine-type recombinase/integrase	-
C5097_RS24555	43263..44168	-	906	Protein_45	IS3 family transposase	-
C5097_RS24560	44233..45383	+	1151	Protein_46	IS30 family transposase	-
C5097_RS24565	45390..46286	+	897	Protein_47	IS3-like element IS600 family transposase	-
C5097_RS24575	46331..46498	-	168	Protein_48	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cofA	1..106432	106432

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11680.49 Da
• Isoelectric Point: 6.4674

>T97287 WP_001159861.1 NZ_CP027106:42394-42699 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINMMFWGI

Nucleotide

• Length: 306 bp

>T97287 NZ_CP027106:42394-42699 [Escherichia coli]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGCCGTTATCGTCTGTTCGTGGATGTGCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGACTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATTGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAATGCCATTAACATGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.39 Da
• Isoelectric Point: 5.0807

>AT97287 WP_000813631.1 NZ_CP027106:42174-42392 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMKNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT97287 NZ_CP027106:42174-42392 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTACTCAAAGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGAAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: T2HS38

Antitoxin

• AlphaFold DB: T2HUI2