Detailed information of TA system TA097264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4384378..4384973
• Replicon: chromosome
• Accession: NZ_CP027103
• Organism: Escherichia coli strain RM14723

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A826P6U8
• Locus tag: C5099_RS22830
• Protein ID: WP_000155160.1
• Coordinates: 4384378..4384755 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A827G8B6
• Locus tag: C5099_RS22835
• Protein ID: WP_000557316.1
• Coordinates: 4384752..4384973 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5099_RS22810	4379954..4381024	-	1071	WP_001357972.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-
C5099_RS22815	4381026..4381871	-	846	WP_000572183.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
C5099_RS22820	4381868..4382755	-	888	WP_000099282.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
C5099_RS22825	4382853..4384169	-	1317	WP_000803589.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
C5099_RS22830	4384378..4384755	-	378	WP_000155160.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C5099_RS22835	4384752..4384973	-	222	WP_000557316.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C5099_RS22840	4385061..4385774	-	714	WP_000416895.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
C5099_RS22845	4385776..4386543	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
C5099_RS22850	4386540..4387817	-	1278	WP_000803800.1	branched chain amino acid ABC transporter permease LivM	-
C5099_RS22855	4387814..4388740	-	927	WP_001295101.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
C5099_RS22860	4388788..4389897	-	1110	WP_001297972.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4379214..4389897	10683

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13945.20 Da
• Isoelectric Point: 6.4830

>T97264 WP_000155160.1 NZ_CP027103:c4384755-4384378 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGLILAANPEFVELTVDVAAGKVSLEDIVTRLRQHGT

Nucleotide

• Length: 378 bp

>T97264 NZ_CP027103:c4384755-4384378 [Escherichia coli]
ATGACCATTCAGTTTATCTCTGCGGAGGAGATAATTTATTTCCACGATAAGTTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTACGAGTATGAAGGCGTTTCCGATCTTT
GGCTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATTTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCCTCATACTTGCTGCAAACCCAGAGTTTGTGGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACACGGAACCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8106.25 Da
• Isoelectric Point: 5.5759

>AT97264 WP_000557316.1 NZ_CP027103:c4384973-4384752 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIMELADK

Nucleotide

• Length: 222 bp

>AT97264 NZ_CP027103:c4384973-4384752 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGACAGAACCTTGCATTGGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCCGCTGTGATTATTAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGATG
CGGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTATGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A826P6U8

Antitoxin

• AlphaFold DB: A0A827G8B6