Detailed information of TA system TA097263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4341988..4342742
• Replicon: chromosome
• Accession: NZ_CP027103
• Organism: Escherichia coli strain RM14723

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A822TTG1
• Locus tag: C5099_RS22635
• Protein ID: WP_021560068.1
• Coordinates: 4342257..4342742 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: C5099_RS22630
• Protein ID: WP_000801915.1
• Coordinates: 4341988..4342266 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5099_RS22625	4339216..4341921	+	2706	WP_000906938.1	HTH-type transcriptional regulator MalT	-
C5099_RS22630	4341988..4342266	+	279	WP_000801915.1	DUF1778 domain-containing protein	Antitoxin
C5099_RS22635	4342257..4342742	+	486	WP_021560068.1	GNAT family N-acetyltransferase	Toxin
C5099_RS22640	4342792..4343820	-	1029	WP_001356914.1	RNA 3'-terminal phosphate cyclase	-
C5099_RS22645	4343824..4345050	-	1227	WP_001105471.1	RNA-splicing ligase RtcB	-
C5099_RS22650	4345269..4345883	+	615	Protein_4147	DNA-binding transcriptional regulator RtcR	-
C5099_RS22655	4345865..4346623	-	759	WP_001296480.1	DeoR/GlpR family transcriptional regulator	-
C5099_RS22660	4346640..4347470	-	831	WP_000928734.1	rhomboid family intramembrane serine protease GlpG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17680.60 Da
• Isoelectric Point: 9.4066

>T97263 WP_021560068.1 NZ_CP027103:4342257-4342742 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSSLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T97263 NZ_CP027103:4342257-4342742 [Escherichia coli]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCAGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGCTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGGGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10450.97 Da
• Isoelectric Point: 4.6336

>AT97263 WP_000801915.1 NZ_CP027103:4341988-4342266 [Escherichia coli]
MKPESKETPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT97263 NZ_CP027103:4341988-4342266 [Escherichia coli]
ATGAAACCGGAAAGCAAAGAAACACCAATCAATATCAGGGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTGGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A822TTG1