Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3801001..3801655 Replicon chromosome
Accession NZ_CP027103
Organism Escherichia coli strain RM14723

Toxin (Protein)


Gene name cptA Uniprot ID A0A2A7MB46
Locus tag C5099_RS19725 Protein ID WP_000244775.1
Coordinates 3801001..3801408 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C5099_RS19730 Protein ID WP_000354046.1
Coordinates 3801389..3801655 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5099_RS19705 3796958..3798691 - 1734 WP_001357613.1 single-stranded-DNA-specific exonuclease RecJ -
C5099_RS19710 3798697..3799407 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C5099_RS19715 3799432..3800328 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C5099_RS19720 3800440..3800961 + 522 WP_001055874.1 flavodoxin FldB -
C5099_RS19725 3801001..3801408 - 408 WP_000244775.1 protein YgfX Toxin
C5099_RS19730 3801389..3801655 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C5099_RS19735 3801898..3802878 + 981 WP_001357615.1 tRNA-modifying protein YgfZ -
C5099_RS19740 3802955..3803614 - 660 WP_000250274.1 hemolysin III family protein -
C5099_RS19745 3803778..3804089 - 312 WP_001357616.1 N(4)-acetylcytidine aminohydrolase -
C5099_RS19750 3804134..3805567 + 1434 WP_001553397.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16008.94 Da        Isoelectric Point: 10.9373

>T97262 WP_000244775.1 NZ_CP027103:c3801408-3801001 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLCLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T97262 NZ_CP027103:c3801408-3801001 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGTGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT97262 WP_000354046.1 NZ_CP027103:c3801655-3801389 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT97262 NZ_CP027103:c3801655-3801389 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A7MB46

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References