Detailed information of TA system TA097249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1876413..1877196
• Replicon: chromosome
• Accession: NZ_CP027103
• Organism: Escherichia coli strain RM14723

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: C5099_RS09560
• Protein ID: WP_032352487.1
• Coordinates: 1876413..1876883 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: C5099_RS09565
• Protein ID: WP_001110447.1
• Coordinates: 1876903..1877196 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5099_RS09540	1871606..1872703	+	1098	WP_000589300.1	flagellar basal body P-ring protein FlgI	-
C5099_RS09545	1872703..1873644	+	942	WP_001304468.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
C5099_RS09550	1873710..1875353	+	1644	WP_001357076.1	flagellar hook-associated protein FlgK	-
C5099_RS09555	1875365..1876318	+	954	WP_001212764.1	flagellar hook-associated protein FlgL	-
C5099_RS09560	1876413..1876883	-	471	WP_032352487.1	GNAT family N-acetyltransferase	Toxin
C5099_RS09565	1876903..1877196	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
C5099_RS09570	1877330..1880515	-	3186	WP_001357075.1	ribonuclease E	-
C5099_RS09580	1881088..1882047	+	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (73-136)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17203.93 Da
• Isoelectric Point: 8.7034

>T97249 WP_032352487.1 NZ_CP027103:c1876883-1876413 [Escherichia coli]
MARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGR
LAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQECLK

Nucleotide

• Length: 471 bp

>T97249 NZ_CP027103:c1876883-1876413 [Escherichia coli]
ATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTGGCTAAAGCAGAGAGCCCTAAAAAA
TCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTTTGGCATATTATTCGCTTGCTTCCA
GCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCGATCCCTGTTGTAGTACTTGGTCGT
CTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGATGCCGGTCTACGAGTACTACAGGC
GGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCCGTGAGTTTTATCTGCGAGTGGGGT
TTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAGGAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT97249 WP_001110447.1 NZ_CP027103:c1877196-1876903 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT97249 NZ_CP027103:c1877196-1876903 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: L4JCW6