Detailed information of TA system TA097245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 721358..721866
• Replicon: chromosome
• Accession: NZ_CP027103
• Organism: Escherichia coli strain RM14723

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: C5099_RS03660
• Protein ID: WP_001534843.1
• Coordinates: 721358..721567 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0G9FLJ7
• Locus tag: C5099_RS03665
• Protein ID: WP_000063155.1
• Coordinates: 721564..721866 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5099_RS03625	717053..717973	+	921	WP_001357522.1	Rpn family recombination-promoting nuclease/putative transposase	-
C5099_RS03630	717956..718279	-	324	WP_001357523.1	winged helix-turn-helix domain-containing protein	-
C5099_RS03635	718457..718621	+	165	WP_001357524.1	DUF1127 domain-containing protein	-
C5099_RS03640	718663..719001	-	339	WP_001357525.1	endoribonuclease SymE	-
-	718996..719072	+	77	NuclAT_5	-	-
-	718996..719072	+	77	NuclAT_5	-	-
-	718996..719072	+	77	NuclAT_5	-	-
-	718996..719072	+	77	NuclAT_5	-	-
-	718996..719072	+	77	NuclAT_6	-	-
-	718996..719072	+	77	NuclAT_6	-	-
-	718996..719072	+	77	NuclAT_6	-	-
-	718996..719072	+	77	NuclAT_6	-	-
C5099_RS03645	719216..719884	-	669	Protein_649	DUF3387 domain-containing protein	-
C5099_RS03650	719878..720051	-	174	Protein_650	SAM-dependent DNA methyltransferase	-
C5099_RS03655	720096..721262	-	1167	WP_000800830.1	restriction endonuclease	-
C5099_RS03660	721358..721567	+	210	WP_001534843.1	BrnT family toxin	Toxin
C5099_RS03665	721564..721866	+	303	WP_000063155.1	BrnA antitoxin family protein	Antitoxin
C5099_RS03670	721900..722856	-	957	WP_001314406.1	GTPase	-
C5099_RS03675	722867..723070	-	204	WP_000467859.1	YbdD/YjiX family protein	-
C5099_RS03680	723188..725337	-	2150	Protein_656	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T97245 WP_001534843.1 NZ_CP027103:721358-721567 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T97245 NZ_CP027103:721358-721567 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11572.05 Da
• Isoelectric Point: 9.4646

>AT97245 WP_000063155.1 NZ_CP027103:721564-721866 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT97245 NZ_CP027103:721564-721866 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0G9FLJ7