Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4257328..4257947 Replicon chromosome
Accession NZ_CP027064
Organism Klebsiella variicola strain WCHKV030666

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CLQ71_RS22365 Protein ID WP_002892050.1
Coordinates 4257729..4257947 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag CLQ71_RS22360 Protein ID WP_008805436.1
Coordinates 4257328..4257702 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ71_RS22345 4252483..4253676 + 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CLQ71_RS22350 4253699..4256845 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
CLQ71_RS22360 4257328..4257702 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
CLQ71_RS22365 4257729..4257947 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CLQ71_RS22370 4258106..4258672 + 567 WP_032438710.1 maltose O-acetyltransferase -
CLQ71_RS28795 4258644..4258772 - 129 Protein_4091 hypothetical protein -
CLQ71_RS22375 4258809..4259279 + 471 WP_008805434.1 YlaC family protein -
CLQ71_RS22380 4259248..4260705 - 1458 WP_016160392.1 PLP-dependent aminotransferase family protein -
CLQ71_RS22385 4260806..4261504 + 699 WP_012968815.1 GNAT family N-acetyltransferase -
CLQ71_RS22390 4261501..4261641 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CLQ71_RS22395 4261641..4261904 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T97213 WP_002892050.1 NZ_CP027064:4257729-4257947 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T97213 NZ_CP027064:4257729-4257947 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT97213 WP_008805436.1 NZ_CP027064:4257328-4257702 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT97213 NZ_CP027064:4257328-4257702 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References